NEUROTOXICITY

PREDICTIVE TOXICOLOGY

Liver, heart, kidney and brain drug-induced toxicities currently account for more than 70% of drug attrition and drug withdrawal. Porsolt together with Fluofarma has developed a range of organ-specific cell-based assays to better predict the toxicity potential of drug candidates prior to in vivo studies.

NEUROTOXICITY

Neurotoxicity represents frequent and troublesome side effects, making the central and peripheral nervous systems important targets of toxicological studies. Fluofarma neurotoxicity assays are based on primary cultures of nerve cells for greater predictivity. Neurotoxic effects are quantified using either automated high-content imaging or live-content imaging, for accurate and fast toxicity screens.

CUSTOM RODENT PRIMARY NEURONAL CULTURES

Fluofarma has a specific expertise in the development of rodent primary nerve cell cultures.

The following primary cultures have already been performed:

> Anterior pituitary gland (rat)

> Cortex (rat and mouse)

> Hippocampus (rat and mouse)

> Hypothalamus (rat)

> Mesencephalon (rat and mouse)

> Striatum (rat and mouse)

> Vomeronasal organ (rat)

> Motoneurons (rat and mouse)

EARLY MARKERS OF NEUROTOXICITY OR TRADITIONAL CYTOTOXICITY ASSAYS

 

> Cell-based assay to monitor early markers of neurotoxicity:

     - neurite outgrowth assays more

     - calcium flux assays more

 

> Standard cytotoxicity assays

     - cytolysis

     - mitochondrial membrane potential 

     more