ONCOLOGY

ONCOLOGY

To support drug discovery projects in oncology, we perform high-content analysis either in cellular models of human tumors in 2D or 3D, or directly in tumor tissues and blood samples. Combining a panel of validated assays for immediate performance and more than 10-year experience in assay development, we assist pharmaceutical companies and academic researchers in the discovery of new cancer drug targets, the identification of new anticancer compounds, and the quantitative analysis of novel cancer biomarkers.

ONCOLOGY READOUTS

CELL PHYSIOLOGY (PROLIFERATION & CELL CYCLE/MIGRATION, ADHESION & DIFFERENTIATION/ANGIOGENESIS)

Clonogenicity

Multiple cellular models

PF 10.4

Cell cycle

Multiple cellular models

PF 3.8

Cell proliferation

Multiple cellular models

PF 3.9

Migration

Multiple cellular models

PF 3.14

Invasion (3D)

Multiple cellular models

PF 3.15

Angiogenesis (endothelial/microendothelial tube formation)

HUVECs/HDMECs Cells/R/6A cells

PF 10.8

c-myc expression

Multiple cellular models

PF 10.25

Pan keratin expression

Multiple cellular models

PF 10.13

CELL STRESS AND METABOLISM

Intracellular reactive oxygen species

Multiple cellular models

PF 3.11

y-secretase activity

Established HeLa cell clone

PF 3.13

Lipid peroxidation

Multiple cellular models

PF 3.18

Glutathione (GSH),
intracellular GSH content

Multiple cellular models

PF 3.28

Hypoxia analysis/quantification

Multiple cellular models

PF 3.20

Heme Oxygenase 1 expression

Multiple cellular models

PF 3.25

MnSOD induction

Multiple cellular models

PF 10.43

Protein-protein interaction

Multiple cellular models

PF 10.28

cAMP quantification

Multiple cellular models

PF 3.27

Calcium signaling

Multiple cellular models

PF 3.33

FLIP expression

Multiple cellular models

PF 10.27

Line-1 expression

Multiple cellular models

PF 10.26

c-myc expression

Multiple cellular models

PF 10.25

Src expression

Multiple cellular models

PF 10.24

FRS phosphorylation

Multiple cellular models

PF 10.23

PLCy phosphorylation

Multiple cellular models

PF 10.21

Cytochrome c release

Multiple cellular models

PF 10.5

Fas expression/phosphorylation

Multiple cellular models

PF 10.16

Protein synthesis quantification

Multiple cellular models

PF 10.46

Lipid, intracellular accumulation:
neutral lipids

Multiple cellular models

PF 3.29

Lipid, intracellular accumulation: phospholipids

Multiple cellular models

PF 3.30

NAD(P)H / FAD

Multiple cellular models

PF 3.31

DNA DAMAGE, CELL DEATH AND APOPTOSIS

NEW !  Immune cell killing

RKO Cell line PF 10.47

Caspase 3/7 activity

Multiple established cell clones PF 3.1
Caspase 3/7 activation Multiple cellular models PF 3.2

Mitochondrial membrane potential

Multiple cellular models

PF 3.3

Cytolysis

Multiple cellular models

PF 3.4

Calpain activity

Multiple established cell clones

PF 3.12

Autophagy (LC3B)

Multiple cellular models

PF 3.17

DNA oxidation

Multiple cellular models

PF 10.6

Histone H3 acetylation (Lys9)

Multiple cellular models

PF 10.19

Micronuclei

Blood

PF 10.22

Caspase 9 activation

Multiple cellular models

PF 10.45

PARP (cleaved)

Multiple cellular models

PF 10.44

dnMT1 expression

Multiple cellular models

PF 10.37

PCNA expression

Multiple cellular models

PF 10.34

Bad expression

Multiple cellular models

PF 10.33

Bcl2 expression

Multiple cellular models

PF 10.32

Bax activation

Multiple cellular models

PF 10.31

Mcl1 expression

Multiple cellular models

PF 10.30

Noxa

Multiple cellular models

PF 10.29

Cytochrome c release

Multiple cellular models

PF 10.5

Apoptosis (Annexin V) quantification

Multiple cellular models

PF 10.2

Caspase 8 activity

Multiple cellular models

PF 10.3

RECEPTOR PHARMACOLOGY AND SIGNALING PATHWAYS

Glucocorticoid receptor subcellular translocation

AtT20 primary rat nuclear translocation pituitary cells

PF 3.26

Androgen receptor nuclear translocation

LnCaP cell line

PF 10.1

Prostate Specific Antigen (PSA) expression LnCap cell line PF 10.15

p21 quantification

Multiple cellular models

PF 10.18

p38 phosphorylation

Multiple cellular models

PF 10.20

p53 expression level/activation

Multiple cellular models

PF 10.17

NF kB activation

Multiple cellular models

PF 3.23

Ras expression

Multiple cellular models

PF 10.14

MEK phosphorylation

Multiple cellular models

PF 10.36

ERK activation (pERK1/2)

Multiple cellular models

PF 3.27

cAMP quantification

Multiple cellular models

PF 3.40

Calcium homeostasis

Multiple cellular models

PF 3.33

STAT1 translocation

Multiple cellular models

PF 3.36

STAT2 translocation

Multiple cellular models

PF 3.35

STAT3 translocation

Multiple cellular models

PF 3.37

STAT5 phosphorylation

Multiple cellular models

PF 10.35

STAT6 phosphorylation

Multiple cellular models

PF 3.34

Poly-Ubiquitin detection

Multiple cellular models

PF 3.28

S6 RP expression/phosphorylation

Multiple cellular models

PF 10.42

c-Jun phosphorylation

Multiple cellular models

PF 10.41

mTOR phosphorylation

Multiple cellular models

PF 10.40

CREB phosphorylation

Multiple cellular models

PF 3.27

PKCα activation

Multiple cellular models

PF 10.39

Rb phosphorylation

Multiple cellular models

PF 10.38

AKT phosphorylation

Multiple cellular models

PF 10.7

FLIP expression

HCT-116 cell line

PF 10.27

Src expression

Multiple cellular models

PF 10.24

FRS2 phosphorylation

MRT4 cell line

PF 10.23

PLCy phosphorylation

Multiple cellular models

PF 10.21

Fas expression/phosphorylation

Multiple cellular models

PF 10.16

Prostate Specific Antigen (PSA) expression

Multiple cellular models

PF 10.15

VHL expression

ACHN cell line

PF 10.10

P130 CAS expression/phosphorylation

ACHN cell line

PF 10.11

FAK expression

ACHN cell line

PF 10.12