PARKINSON'S DISEASE

NEUROBIOLOGY

Fluofarma's contract research services in neuroscience focus on central nervous system (CNS) disorders. We offer to validate drug targets and test neuroactive compounds through customized cell-based assays & quantitative biomarker analysis in brain tissues. Our cell-to-tissue approach delivers solid preclinical proof-of- concepts for CNS drug development.

PARKINSON'S DISEASE

Fluofarma set up a toolbox to support drug discovery projects targeting Parkinson's disease and related disorders. On one hand, we offer to identify and characterize neuroprotective compounds in predictive in vitro models of Parkinson's disease, based on primary neuronal cultures. On the other hand, we developed capacities for the assessment of in vivo drug efficacy and mechanism of action, combining behavioral tests and automated tissue imaging.

IN VITRO MODELS OF PARKINSON'S DISEASE

Neurite outgrowth impairment in in vitro pharmacological models of Parkinson’s disease is a relevant model to assess the degeneration of the nigro-striatal pathway.

Our strategy to evaluate the neuroprotective effects of drug candidates acting upstream of dopaminergic neuronal loss comprises:

> A predictive cellular model: Primary rat mesencephalic culture in microplate format

> Pharmacological treatment: 6-OHDA or rotenone, to induce neurite degeneration

> Quantification of neuritic growth level in the DA neuron population by high-content imaging

IN VIVO MODELS OF PARKINSON'S DISEASE

Through our mother company PORSOLT, expert in parkinsonism and related disorders, we offer to assess the in vivo efficacy of neuroprotective compounds.

Tremor
Tacrine-induced tremulous jaw-movements (TJM)
Rat CNS 10.1
Turning behavior
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.7
Stepping behavior
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.2
L-DOPA dyskinesia
Unilateral medial forebrain bundle (MFB) 6-OHDA lesion
Rat CNS 10.5
Cognitive deficit
Bilateral striatal 6-OHDA lesion
Rat CNS 10.9
Psychosis-like-behaviour
Bilateral striatal 6-OHDA lesion
Rat CNS 10.12
Cognitive, functional, and behavioral tests

BRAIN TISSUES ANALYSIS

Following behavioral and cognitive tests, we offer to analyze brain tissues at the single-cell level using automated tissue imaging, so as to understand the molecular underpinnings of compounds' effects:

> Automated high-content analysis of brain tissues (biomarker quantification with subcellular resolution)