NEURO-ONCOLOGY

NEUROBIOLOGY

Fluofarma's contract research services in neuroscience focus on central nervous system (CNS) disorders. We offer to validate drug targets and test neuroactive compounds through customized cell-based assays & quantitative biomarker analysis in brain tissues. Our cell-to-tissue approach delivers solid preclinical proof-of- concepts for CNS drug development.

NEURO-ONCOLOGY

Fluofarma offers to screen and to characterize anti-cancer drugs in predictive in vitro models of brain tumors. We also complement in vivo studies with automated brain tumor histopathology services, to assess in vivo drug efficacy and drug mechanism of action at the subcellular level.

IN VITRO MODELS OF HUMAN BRAIN TUMORS

Several in vitro brain tumor models are available at Fluofarma to study the underlying mechanisms of tumor development and to test new anticancer drugs:

> Human neuroblastoma cell line (SH-SY5Y)

 

> Human glioblastoma cell lines (DBTRG-O5MG, U-87 MG)

 

Standard cell differentiation procedures can be implemented to induce the expression of a functionally mature neuronal phenotype, as in the case of SH-SY5Y cells.

HIGH-CONTENT HISTOPATHOLOGY OF BRAIN TUMORS

Iba1 and H&E stainings highlight microglia infiltration and reactive astrogliosis within the tumor region in mouse glioblastoma  model.

Iba1 and H&E stainings highlight microglia infiltration and reactive astrogliosis within the tumor region in mouse glioblastoma model.

Fluofarma applies high-content analysis methodologies to brain tumor tissue samples to support in vivo studies in neuro-oncology. Paraffin-embedded tumor tissues, either in tissue section or tissue microarray format, are analyzed by automated tissue imaging, to investigate the in vivo effect and mechanism of action of drug candidates at the molecular level:

> Tissue microarrays approach: to analyze simultaneously large series of brain tissue samples

 

> Automated and multiplexed tissue imaging of immunostained tissue sections and tissue microarrays (IHC/IF)

 

> Quantitative histological analysis: multiplexed analysis of tissue structure detection, biomarker expression levels and subcellular distribution.