BRAIN TISSUE ANALYSIS

NEUROBIOLOGY

Fluofarma's contract research services in neuroscience focus on central nervous system (CNS) disorders. We offer to validate drug targets and test neuroactive compounds through customized cell-based assays & quantitative biomarker analysis in brain tissues. Our cell-to-tissue approach delivers solid preclinical proof-of- concepts for CNS drug development.

BRAIN TISSUE ANALYSIS

HIGH-CONTENT HISTOLOGY OF BRAIN TISSUES

To study biomarkers and their topographic distribution, Fluofarma couples tissue microarray (TMA) technology with automated tissue imaging. Series of tissue specimens are assembled in the same TMA and analyzed simultaneously, thus ensuring optimal and reproducible results. Fluofarma‘s in-house capacities include:

> Brain tissue sampling & processing

> Brain TMA construction

> Multiplexed immunostaining (immunofluorescence or immunohistochemistry detection)

> Automated  imaging of tissue sections or TMAs

> Quantitative biomarker analysis: multiplexed quantification of biomarker expression levels and subcellular localization using dedicated image segmentation and data analysis software applications.

TISSUE MICROARRAY

- Simultaneous processing of large experimental series
- Reduction of variability between specimens
- Standardization and optimization of biomarker staining (IF/IHC)
- Automated acquisition of stained TMA
- Fast identification and true quantification of biomarkers of interest
- TMA construction from a large variety of tissue materials

OVERVIEW OF BRAIN TISSUE PROCESSING