NEUROBIOLOGY

NEUROBIOLOGY

Fluofarma's contract research services in neuroscience focus on central nervous system (CNS) disorders. We offer to validate drug targets and test neuroactive compounds through customized cell-based assays & quantitative biomarker analysis in brain tissues. Our cell-to-tissue approach delivers solid preclinical proof-of- concepts for CNS drug development.

NEUROBIOLOGY READOUTS

Neurite outgrowth

Primary neurons & neuronal cell lines

PF 3.6

γ-secretase activity

Established HeLa cell clone

PF 3.13

Prolactin release

Rat primary pituitary cells

PF 9.9

Microtubule dynamics in cellulo

Primary neurons & neuronal cell lines

PF 9.2

Autophagy (LC3B)

Multiple cellular models

PF 3.17

Proteasome 20S subunit quantification

Multiple cellular models

PF 9.8

Lipid peroxidation

Multiple cellular models

PF 3.18

Nrf2 activation

Multiple cellular models

PF 3.19

Tau Phosphorylation

Rat primary cortical neurons

PF 9.3

NEW ! Amyloid Beta1-42 induced neurotoxicity

Rat primary cortical neurons

PF 9.10

NEW ! Bacterial particles phagocytosis

Rat primary cortical neurons

PF 9.11

NF kB activation

Multiple cellular models

PF 3.23

Glucocorticoid receptor subcellular translocation

AtT20/Primary pituitary cells

PF 3.26

ERK activation (pERK1/2)

Multiple cellular models

PF 3.27

cAMP quantification

Multiple cellular models

PF 3.34

Calcium signaling (ionotropic and metabotropic receptor pharmacology)

Ionotropic and metaotropic receptor pharmacology

PF 3.33

Poly-Ubiquitin detection

Multiple cellular models

PF 3.28

Heme Oxygenase 1 expression

Multiple cellular models

PF 3.25

FKBP52

Multiple cellular models

PF 9.6

CREB phosphorylation

Multiple cellular models

PF 3.35

NeuroToxicity: gliosis

Primary cultures

PF 9.7

Neuromuscular junction quantification

Co-culture of mouse myotubes and primary spinal cord neurons

PF 9.4

Caspase 3/7 activity

Multiple established cell clones

PF 3.1

Caspase 3/7 activation

Multiple cellular models

PF 3.2

Mitochondrial Membrane Potential

Multiple cellular models

PF 3.3

Cytolysis

Multiple cellular models

PF 3.4

Intracellular reactive oxygen species

Multiple cellular models

PF 3.11

Calpain activity

Multiple established cell clones

PF 3.12

NQO1 expression

Multiple cellular models

PF 3.24

Monoamine oxidase B activity

HepG2 cell line

PF 9.1

Myelin Basic Protein (MBP) quantification

Multiple cellular models

PF 9.5