NEUROBIOLOGY

NEUROBIOLOGY

Fluofarma's contract research services in neuroscience focus on central nervous system (CNS) disorders. We offer to validate drug targets and test neuroactive compounds through customized cell-based assays & quantitative biomarker analysis in brain tissues. Our cell-to-tissue approach delivers solid preclinical proof-of- concepts for CNS drug development.

PRIMARY NERVE</br>CELL CULTURES

PRIMARY NERVE
CELL CULTURES

NEUROSCIENCE CELL-BASED ASSAYS

NEUROSCIENCE CELL-BASED ASSAYS

ALZHEIMER'S DISEASE & TAUOPATHY

ALZHEIMER'S DISEASE & TAUOPATHY

PARKINSON'S </br>DISEASE

PARKINSON'S
DISEASE

IN VIVO NEUROSCIENCE SERVICES

IN VIVO NEUROSCIENCE SERVICES

BRAIN TISSUE </br>ANALYSIS

BRAIN TISSUE
ANALYSIS

NEURO</br>ONCOLOGY

NEURO
ONCOLOGY

NEUROBIOLOGY READOUTS

Neurite outgrowth

Primary neurons & neuronal cell lines

PF 3.6

γ-secretase activity

 Established HeLa cell clone

PF 3.13

Prolactin release

 Rat primary pituitary cells

PF 9.9

Microtubule dynamics in cellulo

 Primary neurons & neuronal cell lines

PF 9.2

Autophagy (LC3B)

 Multiple cellular models

PF 3.17

Proteasome 20S subunit quantification

 Multiple cellular models

PF 9.8

Lipid peroxidation

 Multiple cellular models

PF 3.18

Nrf2 activation

 Multiple cellular models

PF 3.19

Tau Phosphorylation

 Rat primary cortical neurons

PF 9.3

NEW ! Amyloid Beta1-42 induced neurotoxicity

 Rat primary cortical neurons

PF 9.10

NEW ! Bacterial particles phagocytosis

 Rat primary cortical neurons

PF 9.11

NF kB activation

 Multiple cellular models

PF 3.23

Glucocorticoid receptor subcellular translocation

 AtT20/Primary pituitary cells

PF 3.26

ERK activation (pERK1/2)

 Multiple cellular models

PF 3.27

cAMP quantification

 Multiple cellular models

PF 3.34

Calcium signaling (ionotropic and metabotropic receptor pharmacology)

 Ionotropic and metaotropic receptor pharmacology

PF 3.33

Poly-Ubiquitin detection

 Multiple cellular models

PF 3.28

Heme Oxygenase 1 expression

 Multiple cellular models

PF 3.25

FKBP52

 Multiple cellular models

PF 9.6

CREB phosphorylation

 Multiple cellular models

PF 3.35

NeuroToxicity: gliosis

 Primary cultures

PF 9.7

Neuromuscular junction quantification

 Co-culture of mouse myotubes and primary spinal cord neurons

PF 9.4

Caspase 3/7 activity

 Multiple established cell clones

PF 3.1

Caspase 3/7 activation

 Multiple cellular models

PF 3.2

Mitochondrial Membrane Potential

 Multiple cellular models

PF 3.3

Cytolysis

 Multiple cellular models

PF 3.4

Intracellular reactive oxygen species

 Multiple cellular models

PF 3.11

Calpain activity

 Multiple established cell clones

PF 3.12

NQO1 expression

 Multiple cellular models

PF 3.24

Monoamine oxidase B activity

 HepG2 cell line

PF 9.1

Myelin Basic Protein (MBP) quantification

 Multiple cellular models

PF 9.5