METABOLIC DISEASES

METABOLIC DISEASES

Metabolism related disorders, such as diabetes and obesity are key therapeutic areas that have attracted global attention over recent years. Fluofarma and Porsolt have in place a comprehensive range of models to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases...

METABOLIC DISEASES READOUTS - CELLULAR METABOLISM

Glycerol release / lipolysis

Adipocytes

PF 7.1

Inositol triphosphate receptor channel function

 H9C2 Cells

PF 3.21

Lipid, intracellular accumulation: neutral lipids

 Multiple cellular models

PF 3.29

Lipid, intracellular accumulation: phospholipids

 Multiple cellular models

PF 3.30

NAD(P)H / FAD

 Multiple cellular models

PF 3.31

Calcium signaling

 Multiple cellular models

PF 3.33

Hypoxia analysis / quantification

 Multiple cellular models

PF 3.20

CELL-BASED ASSAYS RELATED TO METABOLIC DISORDERS

 

>  in vitro models based on primary cell cultures or cell lines: adipocytes, hepatocytes, pancreatic cells, etc...

>  Metabolism assays: lipolysis assays, lipids and phospholipids accumulation, lipid peroxidation, LDL uptake, NADH/FAD content, ATP content, intracellular ROS, P-glycoprotein (P-gp) activity, intracellular Glutathione (GSH) content, hepatobiliary function, ...

>  Neuroendocrinology-related assays: prolactin release and dopamine metabolism, glucocorticoid receptor translocation, etc...

>>> Check our general assay portfolio

IN VIVO SERVICES FOR METABOLIC SYNDROME

 

Through PORSOLT capabilities, we offer to perform in vivo studies in predictive models of metabolic syndrome:

> Genetic and diet-induced in vivo models of obesity (Ob/Ob genetic model and high fat diet-induced obesity model) and diabetes (Db/Db genetic model and high fat diet-induced diabetes model)

> LC-MS/MS analysis: Detection of metabolic factors, cytokines, etc., on tissue lysate or blood & derivatives (PBMC & blood samples, monocytes, macrophages,...)

> High content histology: Automated tissue imaging and multiplexed analysis of key markers with single-cell resolution (biomarker expression levels & subcellular localization).

ADDITIONAL CAPABILITIES