Metabolism related disorders, such as diabetes and obesity are key therapeutic areas that have attracted global attention over recent years. Fluofarma and Porsolt have in place a comprehensive range of models to assist with the development of novel therapeutic agents for the treatment and reduction of risk factors associated with metabolic diseases...
CELL-BASED ASSAYS RELATED TO METABOLIC DISORDERS
> in vitro models based on primary cell cultures or cell lines: adipocytes, hepatocytes, pancreatic cells, etc...
> Metabolism assays: lipolysis assays, lipids and phospholipids accumulation, lipid peroxidation, LDL uptake, NADH/FAD content, ATP content, intracellular ROS, P-glycoprotein (P-gp) activity, intracellular Glutathione (GSH) content, hepatobiliary function, ...
> Neuroendocrinology-related assays: prolactin release and dopamine metabolism, glucocorticoid receptor translocation, etc...
IN VIVO SERVICES FOR METABOLIC SYNDROME
Through PORSOLT capabilities, we offer to perform in vivo studies in predictive models of metabolic syndrome:
> Genetic and diet-induced in vivo models of obesity (Ob/Ob genetic model and high fat diet-induced obesity model) and diabetes (Db/Db genetic model and high fat diet-induced diabetes model)
> LC-MS/MS analysis: Detection of metabolic factors, cytokines, etc., on tissue lysate or blood & derivatives (PBMC & blood samples, monocytes, macrophages,...)
> High content histology: Automated tissue imaging and multiplexed analysis of key markers with single-cell resolution (biomarker expression levels & subcellular localization).
METABOLIC DISEASES READOUTS - CELLULAR METABOLISM
Glycerol release / lipolysis |
Adipocytes |
PF 7.1 |
Inositol triphosphate receptor channel function |
H9C2 Cells |
PF 3.21 |
Lipid, intracellular accumulation: neutral lipids |
Multiple cellular models |
PF 3.29 |
Lipid, intracellular accumulation: phospholipids |
Multiple cellular models |
PF 3.30 |
NAD(P)H / FAD |
Multiple cellular models |
PF 3.31 |
Calcium signaling |
Multiple cellular models |
PF 3.33 |
Hypoxia analysis / quantification |
Multiple cellular models |
PF 3.20 |