Starting with a core expertise in polarized primary cultures of rat hepatocytes, Fluofarma set up a tool box to support to support drug discovery in hepatology. From cell-based screening up to biomarker studies in liver tissues, we offer quantitative and automated approaches to characterize the effects of drug candidates on liver functions and explore their mechanism of action (MOA).
> in vivo-like features: drug metabolizing-enzymes, polarized phenotype, bile canaliculi networks
> in vitro liver disease models based pharmacological treatment
> Screening format: microplates
>>> High-biological relevance, fast turnaround
> No more hidden drug effects: kinetic quantification of drug effects on hepatocyte viability or bile canaliculi structure over several days.
> Accurate compound profiling: generation of time-dependent EC50 values.
>>> Phenotypic screening & drug profiling
> Functional assays: Lipid accumulation, hepatobiliary function, redox state, LDL uptake, NADH/FAD content, intracellular Ca2+, mitochondrial function, ...
> Cell signaling assays: NQO1, HO1, MRP2, GSH, p53, caspase 3/7 ...
> Generation of in vivo models of liver pathologies
> High-content analysis of liver tissue microarrays
> Automated and multiplexed biomarker quantification
>>> In vivo proof of drug efficacy / safety / mechanism of action
LDL uptake |
HepG2 cell line |
PF 3.10 |
Cholestasis / Bile canaliculi network |
Primary hepatocytes - sandwich configuration - hepatic tissue |
PF 3.16 |
Glutathione (GSH), intracellular GSH content |
Multiple cellular models |
PF 3.28 |
Acetaminophen toxicity model (GSH contents, cytolysis) |
Mouse primary hepatocytes |
PF 3.41 |
Steatosis/Lipid, intracellular accumulation: neutral lipids |
Multiple cellular models |
PF 3.29 |
NEW ! NASH model Intracellular lipid accumulation |
Primary Human and primary mouse hepatocytes |
PF 6.1 |
Lipid, intracellular accumulation: phospholipids |
Multiple cellular models |
PF 3.30 |
Mitochondrial Membrane Potential |
Multiple cellular models |
PF 3.3 |
MRP2 expression |
Primary hepatocytes - sandwich configuration - hepatic tissue |
PF 3.5 |
Cytolysis |
Multiple cellular models |
PF 3.4 |
NQO1 expression |
Multiple cellular models |
PF 3.24 |
Heme Oxygenase 1 expression |
Multiple cellular models |
PF 3.25 |
NAD(P)H / FAD |
Multiple cellular models |
PF 3.31 |