SKIN PROTECTION - Against pollution

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

SKIN PROTECTION - Against pollution

Almost 80% of urban areas have levels of pollutants greater than the recommended levels of the World Health Organization (WHO). Given that 54% of the world population lives in urban areas, the number of people exposed to pollutants continues to increase, especially in big cities. Anti-pollution objectives are currently on top of the cosmetic industry agenda.

FLUOFARMA offers many in vitro models to evaluate:
- Cell viability measurement (NHDF, NHEK, HaCaT)
- Autophagy which is a key system to protect against pollution exposition (LC3B)
- Inflammatory cytokine release
- Reactive Oxygen species (ROS) and Lipid peroxidation measurement
- DNA damages

Protection against ROS generation and lipid peroxidation induced by pollutant

Numerous epidemiological studies have established a consistent association between urban dust particulate matter concentrations and increased skin troubles. Various experimental studies provide a correlation between urban dust oxidative capacity and its toxicity. Oxidative stress can damage lipids, proteins, membrane, DNA, and can cause cell death by necrotic or apoptotic processes.

FLUOFARMA provides new assays to assess anti-pollution claim by challenging ingredients against ROS production (left graph) and lipid peroxidation (right graph) induced by urban dust in human keratinocytes.

Autophagy modulation in Hela cells

The importance of autophagy process in a number of pathologies, as well as in the aging process or protection against pollution is now an emerging trend. Autophagy modulation is measured using the LC3B staining (early marker of autophagosome). HeLa cells are seeded in 96-well plates and treated the day after with PP242 or medium for 3h, and then fixed and stained for LC3B. The mean number of LC3B positive foci per cells is measured (Nucleus – blue staining ; LC3B foci – green staining).