SKIN MICROCIRCULATION

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

SKIN MICROCIRCULATION

The skin is richly supplied with a vascular network, largely exceeding its nutritive requirements.  Skin microcirculation is a fundamental actor involved in thermoregulation, wound healing, immune reactions and the control of blood pressure. It is also a major actor of skin redness, rosacea and discreet erythema.

FLUOFARMA offers many in vitro models to evaluate :

- Angiogenesis and neovascularization stimulation (HUVEC / NHDF co-culture)
- Endothelial cells signaling (AMPc and calcium measurement)
- Protection of endothelial cells against stress (…)

Evaluation CGRP receptors antagonists

A functional assay assessing antagonistic properties of compounds on endogenous CGRP receptors, activated with the potent vasodilator CGRP (Calcitonin Gene-Related Peptide). Primary Human Dermal Microvascular Endothelial Cells were seeded in 96-well plates. Since CGRP receptors are Gs-coupled GPCRs, cAMP accumulation was quantitated, by HTRF. The dose-dependent aCGRP-induced cAMP production was rightward-shifted by the reference antagonist CGRP 8-37, indicating inhibition.

Evaluation of angiogenesis (NHDF/HUVEC)

Angiogenesis processes (tube formation and tube length quantification) can be monitored in a 96-well plate format. For example a co-culture of normal human dermal fibroblast (NHDF) and GFP-labeled human umbilical vein endothelial cells (HUVEC) is performed (Essen bioscience kit). Tube length is kinetically monitored for 11 days in-vitro using VEGF as pro-angiogenic control and Axitinib as anti-angiogenic reference.