HISTOLOGY & BIOMARKER SERVICES

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

HISTOLOGY & BIOMARKER SERVICES

Our automated approach to histology provides highly reproducible, multiplexed and quantitative biomarker studies as well as data to illustrate your compound’s effects :

- Tissue sourcing: automated dehydration and embedding in paraffin of ex-vivo explants, Reconstituted Human Epidermis or skin biopsies.
- Staining can be performed for any protein expression : IHC staining or any other dyes (Hematoxylin/Eosin for structural analysis, Sirius Red for Collagen expression … as well as multiplexed fluorescence.
- Automation: automated imaging and cytometric segmentation of IF/IHC-stained tissue sections or Tissue Micro Arrays.
- Multiplexed biomarker quantification: quantitative biomarker scoring with spatial information at the single-cell level (biomarker intracellular localization, subcellular and cellular morphological features) allowing subpopulation analyses.

Human skin staining

FLUOFARMA is able to handle, stain (IHC or multiplex in IF) and quantify biomarkers with spatial information at the single-cell level allowing subpopulation analyses in Human skin explant. For example :

- Morphology of skin tissue such as epidermal thickness, morphology of epidermis layers Hematoxylin Eosin
- Cell to cell adhesion E-Cadherin
- Barrier function of the skin through Loricrin and filaggrin staining
- Proliferation markers to study tissue regeneration or uncontrolled proliferation Ki67

Hematoxylin Eosin

Hematoxylin Eosin

E-Cadherin

E-Cadherin

Ki67

Ki67

Loricrin

Loricrin

Nucleus (blue) Loricrin (red) Filaggrin (green)

Nucleus (blue) Loricrin (red) Filaggrin (green)

Reconstituted Human Epidermis staining

FLUOFARMA is able to handle, stain and quantify biomarkers with spatial information at the single-cell level, allowing for subpopulation analyses in Reconstituted Human epidermis. For example :

- Morphology of skin tissue such as epidermal thickness, morphology of epidermis layers Hematoxylin Eosin
- Cell to cell adhesion E-Cadherin
- Barrier function of the skin through Loricrin and filaggrin staining
- Proliferation markers to study tissue regeneration or uncontrolled proliferation Ki67

Hematoxylin Eosin

Hematoxylin Eosin

E-Cadherin

E-Cadherin

Ki67

Ki67

Loricrin

Loricrin

Nucleus (blue) Loricrin (red) Filaggrin (green)

Nucleus (blue) Loricrin (red) Filaggrin (green)

Skin Biomarker Quantification

Processing of IHC images using color and texture information. Once the layer of interest inside the skin has been identified, we can compute several readouts such as cell count or thickness. For example :

- Loricrin staining: Loricrin area is extracted using color features to compute its thickness
- Ki67 staining: Cell counting inside the epidermis and identification of the Ki67 sub-population e.g. Percentage of Ki67 positive cells inside the epidermis (brown stained cells)

Epidermis (green), Negative cells (blue), Ki67+ cells (red)

Skin Layers Identification & Quantification

Color and Texture information and Supervised Machine Learning algorithms can be used to identify the different skin layers of Hematoxylin Eosin images inside a database. It is possible to quantify the morphology of skin tissue such as epidermal thickness, percentage, etc.