EPIDERMAL & DERMAL REGENERATION RESTRUCTURATION

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

EPIDERMAL & DERMAL REGENERATION RESTRUCTURATION

The epidermis is a continually renewing stratified, squamous epithelium (90-95% keratinocytes) which relies on proper cell proliferation and differentiation in order to maintain or to restore epidermal structure and functions. Fibroblasts also have a key role to play in the upkeep of connective tissue mainly composed of extracellular matrix.

FLUOFARMA offers many in vitro models to evaluate :

- Migration in “wound healing” of human keratinocytes (NHEK, HaCaT)
- Migration / proliferation of Human dermal fibroblasts (NHDF)
- Viability, metabolism and cell morphology (NHEK, NHDF, L929)
- Collagen synthesis in Human dermal fibroblasts (NHDF)

Evaluation of HaCaT migration

Fluofarma can provide a high throughput assay of cell migration. Using an Incucyte® Wound Maker we can perform identical wounds in all 96 wells at the same time and follow kinetically the evolution of the wound (migration or proliferation depending on assays conditions) to best measure the effects of a compound.

Evaluation of primary adult keratinocytes migration

Fluofarma can provide a high throughput assay of NHEK migration (adult Normal Human Epidermal Keratinocytes). Using an Incucyte® Wound Maker we can perform identical wounds in all 96 wells at the same time and follow kinetically the evolution  of the wound (migration or proliferation depending on assays conditions) to best measure the effects of a compound.

Evaluation of NHDF migration and proliferation

Measurement of both migration and proliferation combined using Normal Human Dermal Fibroblast (NHDF) cells plated in 96-well plates in medium without serum. The confluent cell layer was wounded and the covering of the wound by cell migration was kinetically monitored and quantified as a cell confluence in the wound. Stimulation with 10% serum was performed as a positive control.