CELL MODELS

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

CELL MODELS

FLUOFARMA offers many in vitro models in 96-well plates format to evaluate your compound on different cell types :

- Immortalized Keratinocytes (HaCaT)
- Primary Keratinocytes from adult or juvenile donors
- Primary Fibroblasts from juvenile donors
- Primary Melanocytes
- Langerhans cells (dendritic cells in the skin) from CD34+ progenitor (umbilical cord blood)
- Peripheral Neurons from Induced pluripotent stem cell (iPSC)
- Human Dermal Microvascular Endothelial Cells (HDMEC)

FLUOFARMA can also handle 3D models using :

- Spheroid formation
- Reconstituted human epidermis
- Human skin explant

Calcium measurement in Melanocytes

Normal Human Epidermal Melanocytes (NHEM), isolated from one adult donor. The donor is a Caucasian woman, 33 years old, with an estimated skin phototype of II/III.

Endothelin-1 was tested at 5 concentrations (1 nM to 100 nM) to assess the ability of the NHEM to respond via an endogenous G Protein Coupled Receptor (GPCR) in a calcium assay.

Anti inflammatory effects on primary human Langerhans cells cytokine release

Dendritic cells (DC) and their immature counterparts, Langerhans cells (LC), are highly specialized antigen-presenting cells located in the skin. DC and LC play a key role in the induction phase of contact allergenicity and other immunological reactions of the body. In skin infections, the local Langerhans cells take up and process microbial antigens to become fully functional antigen-presenting cells.

Fluofarma offers a human primary dendritic cell model with high Langerhans cells purity, more than 75% of cells express the langerin marker (localized in the Birbeck granules, specific organelles present in the cytoplasm of Langerhans cells). Langerhans cells were able to respond to LPS and PMA stimuli by cytokine liberation (IL-6, IL-8 and TNFa) which was inhibited by the application of a TLR4 Inhibitor (Tak-242).

Evaluation CGRP receptors antagonists

A functional assay assessing antagonistic properties of compounds on endogenous CGRP receptors, activated with the potent vasodilator CGRP (Calcitonin Gene-Related Peptide). Primary Human Dermal Microvascular Endothelial Cells were seeded in 96-well plates. Since CGRP receptors are Gs-coupled GPCRs, cAMP accumulation was quantitated, by HTRF. The dose-dependent aCGRP-induced cAMP production was rightward-shifted by the reference antagonist CGRP 8-37, indicating inhibition.