ANTI-AGING & FIRMING ACTIVITY

COSMETIC TESTING

Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

ANTI-AGING & FIRMING ACTIVITY

Skin aging is a complex biological process influenced by a combination of endogenous and exogenous / extrinsic factors. A lot of skin anti-aging strategies attempted to reverse the dermal and epidermal signs of ageing by blocking exposome damages or boosting dermal / epidermal regeneration. FLUOFARMA offers in vitro models to evaluate products or active ingredients :

- Cell proliferation, migration (NHDF, HaCaT)
- Protection against oxidative stress (NHEK, HaCaT) 
- Collagen synthesis (NHDF)
- GSH / ROS and proteasome (20S) measurements
- Senscence measurement

We can also stain Reconstructed human epidermis or ex-vivo human skin explants for :

- Morphology of skin tissue such as epidermal thickness, morphology of epidermis layers
- Proliferation (KI-67)

Senescence measurement for anti-aging claims

Senescence can be induced by using low concentrations and short exposure to hydrogen peroxide. Cells exhibit senescent-morphological features, and senescence-associated b-galactosidase positivity (4 times increase – left graph) which can be correlated with a diminution in their ability to invade the wound in wound healing assay (left graph). Anti-aging ingredients can be challenged against this model to show i) decrease in induced-senescence  ii) stimulation of senescent-keratinocyte (e.g. EGF in right graph).

Collagen synthesis in NHDF

Normal Human Dermal Fibroblasts (NHDF) were seeded in 96-well plates. After treatment with reference compound (Ascorbic acid at 300 µM and TGF-beta at 30 ng/mL for 72h), the total collagen at the level of the cell layer was quantified with Sirius Red staining and absorbance measurement.