Because visual scoring of tissues can lack predictive power when slight variations in biomaker expression occur, or when multiplexed studies are required, Fluofarma transferred its expertise in high-content analysis to analyze tissue samples at the single-cell level. In the context of preclinical studies and clinical trials, our automated approach to histology results in highly reproducible, multiplexed and quantitative biomarker studies.
> Miniaturization: tissue microarray approach (hundreds of tissue spots on a single histologic slide) for fast and uniform analysis of large collections of tissue samples.
> Automation: automated imaging and cytometric segmentation of IF/IHC-stained tissue sections or TMAs.
> Multiplexed biomarker quantification: quantitative biomarker scoring (endogenous expression level) with spatial information at the single-cell level (biomarker intracellular localization, subcellular and cellular morphological features) allowing subpopulation analyses.
> Tissue sourcing: tissue isolation following in vivo experiments, access to archival tumor tissue libraries (patient clinical data provided).
> Custom tissue microarrays: in-house construction of paraffin-embedded tissue microarrays using artificial 3D microtissues, and tissue samples from animals or patients.
> Cytometric image & data analysis: custom image segmentation and multi-dimensional data analysis from subcellular level up to tissue architecture using proprietary software applications.
INSTRUMENT | TissueFAXS system (TissueGnostics) |
TISSUE SAMPLES | Artificial 3D microtissues, archival tissues, tissue samples from in vivo experiments and clinical trials |
TISSUE FORMAT | Paraffin-embedded tissue sections or tissue microarrays (TMA) |
READOUTS | Cellular and subcellular morphological features, tissue architecture & immuno-stained markers (IF/IHC) |