HIGH CONTENT HISTOLOGY

HIGH CONTENT HISTOLOGY

Because visual scoring of tissues can lack predictive power when slight variations in biomaker expression occur, or when multiplexed studies are required, Fluofarma transferred its expertise in high-content analysis to analyze tissue samples at the single-cell level. In the context of preclinical studies and clinical trials, our automated approach to histology results in highly reproducible, multiplexed and quantitative biomarker studies.

KEY ADVANTAGES OF AUTOMATED HIGH-CONTENT HISTOLOGY

> Miniaturization: tissue microarray approach (hundreds of tissue spots on a single histologic slide) for fast and uniform analysis of large collections of tissue samples.

> Automation: automated imaging and cytometric segmentation of IF/IHC-stained tissue sections or TMAs.

> Multiplexed biomarker quantification: quantitative biomarker scoring (endogenous expression level) with spatial information at the single-cell level (biomarker intracellular localization, subcellular and cellular morphological features) allowing subpopulation analyses.

FLUOFARMA'S ADDED VALUE

> Tissue sourcing: tissue isolation following in vivo experiments, access to archival tumor tissue libraries (patient clinical data provided).

> Custom tissue microarrays: in-house construction of paraffin-embedded tissue microarrays using artificial 3D microtissues, and tissue samples from animals or patients.

> Cytometric image & data analysis: custom image segmentation and multi-dimensional data analysis from subcellular level up to tissue architecture using proprietary software applications.

TECHNICAL FEATURES

INSTRUMENT TissueFAXS system (TissueGnostics)
TISSUE SAMPLES Artificial 3D microtissues, archival tissues, tissue samples from in vivo experiments and clinical trials
TISSUE FORMAT Paraffin-embedded tissue sections or tissue microarrays (TMA)
READOUTS Cellular and subcellular morphological features, tissue architecture & immuno-stained markers (IF/IHC)