FLOW CYTOMETRY

FLOW CYTOMETRY

Fluofarma holds a specific expertise in the multiplexed detection of intracellular markers by automated flow cytometry. Therefore, in addition to supporting drug discovery through high-throughput flow cytometry screens, we offer to quantify biomarkers in blood samples in the context of in vivo studies and clinical trials, to assess target hitting, perform pharmacokinetic studies or stratify patients.

KEY ADVANTAGES OF FLOW CYTOMETRY


> High-throughput: up to 40 000 events acquired per second

> Multiplexing: up to 18 parameters measured simultaneously

> Single-cell analysis: immunophenotyping enables the analysis of intracellular events in specific cell subpopulations

> Robust measurements, with accurate Z’ factors

FLUOFARMA'S ADDED VALUE


> Predictive cellular models tailored to your need, based on cell linesprimary cell cultures or stem cells

> Proprietary DAP technology: for reagentless quantification of caspase, calpain or γ-secretase activity by flow cytometry

> Key expertise in intracellular markers (phosphoproteins, signaling pathways, ...) for cell-based screening and biomarker detection in blood samples

TECHNICAL FEATURES

INSTRUMENTATION  BD Fortessa Flow Cytometer
MULTIPLEXING 3-laser flow cytometer: up to 18 parameters analyzed simultaneously
BIOLOGICAL SAMPLES cell cultures, blood samples 
SAMPLE FORMAT 96- / 384-well plates