Purpose of the study: Quantification of fibrosis in a Non-Alcoholic Steatohepatitis (NASH) rat model.


A color deconvolution method has been used to extract the collagen region inside Sirius Red images. A final segmentation, obtained using a binarisation technique, allows to quantify the percentage of fibrosis.


Quantitative readouts for fibrosis (additional readouts can be computed according to the needs of the client):

Percentage of fibrotic area

Total area of liver tissue

On this model, additional quantifications can be computed such as steatosis.











Quantitative readouts for steatosis:

Steatosis area Lipid droplet size
Histogram of lipid droplet size