Purpose of the study: Quantification of fibrosis in a Unilateral Ureteral Obstruction renal fibrosis rat model.


A color deconvolution method has been used to extract the collagen region inside Sirius Red images. A final segmentation, obtained using a binarisation technique, allows to quantify the percentage of fibrosis.


Quantitative readouts for fibrosis (additional readouts can be computed according to the needs of the client):

Percentage of fibrotic area

Total area of kidney tissue