CELL-BASED ASSAY DEVELOPMENT

Fluofarma is at first a cell biology company. When we engage in the development of a new cell-based assay, our prime focus is on the design of a relevant and predictive cellular model. We aim at testing compounds in in vitro models that closely mimic in vivo conditions. Our second priority is to optimize assays for automated high-content screening, enabling robust and quantitative data analysis at the single cell level.

CUSTOMIZED CELLULAR MODELS

Starting from the pathophysiological context you’re interested in, we custom-design an in vitro cellular model in 96-/384-well format for high-throughput cell-based screening.

- In-house preparation of primary cell cultures

- Mono- and multi-cell type culture systems

- 2D or 3D  configuration based on cell lines, primary cells or differentiated stem cells. 

- in vitro disease models: pharmacological or genetically generated

RELEVANT PHENOTYPIC & MOLECULAR READOUTS

We hold more than 10-year-experience in assay development across a wide range of drug targets, functional responses, intracellular localizations, upstream or downstream activation markers.In each case, we select the most relevant and robust readout to highlight the specific action of compounds.For better compound profiling, and to reduce turnaround time, we favor multiplexed readouts.

> Phenotypic readouts: Morphological changes

- Angiogenesis
- Neurite outgrowth
- Invasion
- Cell proliferation / migration

> Molecular readouts

- Molecular signaling pathways
- Calcium signaling
- cAMP response
- Transduction signal response
- Metabolic activities
- Relocalization & translocation
- Cell secretion
- Protein expression / phosphorylation                  Learn more

ASSAY MINIATURIZATION AND AUTOMATION

Newly-developed assays are miniaturized and adapted to automated high-content analysis platforms.

Newly-developed assays are miniaturized and adapted to automated high-content analysis platforms.

We optimize assays to generate robust and quantitative data at high-throughput, while minimizing compound and reagent consumption. Assays are miniaturized, multiplexed and adapted to a relevant platform, for automated signal analysis:

- Live-content imaging

- High-throughput flow cytometry

- High-content imaging

- High-throughput fluorometric imaging plate reader (FLIPR Tetra®)

- High-content histology

IMAGE & DATA ANALYSIS

In the context of assay development projects, our image & data analysis team can develop custom algorithms & sofware applications to make the most of your data:

- Image segmentation, signal quantification, feature extraction

- Analysis and management of large data loads