CELL STRESS

Cells respond both to normal metabolic signaling to promote growth and to exogenous stressors through a number of adaptive stress responses pathways.

Fluofarma has developed a selection of cell-based assays to measure the activation/inactivation of cell stress pathways, using rapid and reliable surrogate reporters. 

Our cell stress assays can be used either to assess the potential toxicity of lead compounds or to study their mechanism of action.

FUNCTIONAL CELL STRESS ASSAYS

To study the effects of drug compounds on cell stress, Fluofarma maintains a range of validated cell-based assays compatible with high-throughput cell-based screening. Each assay can be adapted to the cellular model of your choice:

> ROS formation assay


> Lipid peroxidation assay


> Glutathione depletion assay


> NADH/FAD assay


> Mitochondrial membrane potential assay


> Calcium homeostasis assay

HIGH-CONTENT ANALYSIS OF CELL STRESS MARKERS

HIF-1α expression and distribution in HCT116 3D microtissue. After immunohistochemistry of HIF-1α multiplexed with H&E staining (upper-left panel) and HIF-1α segmentation mask (lower-left panel), analysis demonstrates HIF-1α accumulation in the center of the spheroid (right panel).

HIF-1α expression and distribution in HCT116 3D microtissue. After immunohistochemistry of HIF-1α multiplexed with H&E staining (upper-left panel) and HIF-1α segmentation mask (lower-left panel), analysis demonstrates HIF-1α accumulation in the center of the spheroid (right panel).

In addition to functional assays, we offer to quantify protein markers involved cellular events such as oxidative stress, genotoxic stress, ER stress... High-content analysis is performed at single-cell level, using automated confocal microscopy and image segmentation applications. We can for instance assess drugs' effects on:

> Nrf-2 signaling (HO1, NQO1...),
> DNA damage signaling (p53, gammaH2AX ...),
> Hypoxia signaling (HIF-1α)