CELL MIGRATION / INVASION & ADHESION

Cell migration is central to key biological functions such as embryogenesis, immune response and inflammation. But it also participates in many disorders such as vascular diseases and cell cancer metastasis.

Fluofarma developed standardized, sensitive and robust cell migration assays to study its underlying mechanisms and test novel therapeutic strategies.

MIGRATION OF ADHERENT CELLS: THE WOUND HEALING ASSAY

 

> No reagents: cell migration is triggered by removing a strip of cells at the center of the well, using a 96-well wound maker tool.

> Kinetic monitoring: cell migration can be monitored over several days by label-free live-content imaging.

> Quantitative: cell migration is quantified by measuring cell density within the wound.

> Cell invasion can be addressed using similar approach based on matrigel matrix

MIGRATION OF NON-ADHERENT CELLS: THE TRANSWELL MIGRATION ASSAY

 

 > 96-well Boyden chamber assay: a gold-standard for in vitro chemotaxis measurements

> High-throughput: total number of cells and the number of migrating cells are quantified by flow cytometry.