CELL GROWTH & PROLIFERATION

Assessing in vitro cell proliferation is a key mean for monitoring cellular health but also for the functional screening of drug candidates. Fluofarma offers different options to identify and characterize compounds either activating or inhibiting cell growth and proliferation.

PROLIFERATION INDEX ASSAY

Fluofarma proliferation index assay is both simple and robust, being based on the dilution rate of a fluorescence membrane dye:

> The dye is compatible with multiplexed analysis

 

> Fast turnaround: assay is performed by high-throughput flow cytometry

> Accurate: dilution rate of fluorescent probe is a direct readout of the number of cell divisions. Data is not affected by variations in cell number per well or cell metabolic activity.

KINETIC CELL PROLIFERATION ASSAY

We use live-content imaging to monitor cell proliferation over time:

> Kinetic & label-free proliferation assay (no reagent needed)

> No hidden drug effects: cells are monitored over several days

> Accurate compound profiling: time-dependent EC50 values

> Multiplexing with cytolysis detection

>>> Cell proliferation can also be assessed by flow cytometry or high-content imaging, based on BrdU/EdU incorporation to label DNA-replicating cells

HIGH-CONTENT ANALYSIS OF CELL GROWTH AND PROLIFERATION MARKERS

We offer to quantify protein markers involved in cell growth and proliferation such as Ki67, Histone H3, p21, PCNA... at the single-cell level in 2D & 3D cellular models, using high-content imaging methodologies.

>>> Learn about our drug discovery services in oncology and approach for predictive toxicology.