CELL CYCLE & DNA DAMAGE

Cell cycle is a highly regulated process of cell growth and division. It is organized in a succession of 4 phases, G0/G1→S→G2→M phases, that are tightly regulated by protein checkpoints in order to guarantee the correct replication of DNA. Insertion of DNA errors or DNA damage will activate cell cycle checkpoints and lead to cell cycle arrest or apoptosis. When such mechanisms are dysregulated, as in cancer, they are of particular interest for therapeutic strategies.

Our cell cycle assays and DNA damage cell-based assays are tailored for high-throughput anti-cancer screening and early toxicity testing.

Available on different high-content analysis platforms and compatible with multiplex screening, they can be performed in the cellular model of your choice.

CELL CYCLE ANALYSIS BY FLOW CYTOMETRY

 

Based on the analysis of cellular DNA content detected by a DNA intercalating agent such as propidium iodide, flow cytometry is a powerful tool to reveal cell distribution in the three major phases of cell cycle, G1 versus S versus G2/M phases.

It also allows for quantification of the percentage of apoptotic cells in the Sub G1 phase.

This approach can be multiplexed with more specific markers such as EdU/BrdU staining to label DNA-replicating cells or key cell cycle markers.

HIGH-CONTENT ANALYSIS OF CELL CYCLE & DNA DAMAGE MARKERS

 

 We offer to use high content imaging to quantify at single cell level some key markers of cell proliferation and measure the status of signaling pathways involved in DNA damage, such as p53 activation, phospho-Rb, γH2AX, phosphorylation or acetylation of histone... 


>>> Learn more about our preclinical services in oncology and predictive toxicology