CALCIUM SIGNALING

Calcium plays an important role in cell signaling, either by direct signal transduction or acting as a second-messenger. Calcium signaling regulates a large range of physiological cell functions and processes such as motility, cell proliferation & apoptosis, excitability & depolarization, exocytosis & synaptic plasticity. In addition, movements of calcium ions that result in membrane potential changes are particularly important in heart function and neuronal transmission.

A CORE EXPERTISE IN CALCIUM SIGNALING & INTRACELLULAR CALCIUM MOBILIZATION

Fluofarma developed core expertise and capacities to monitor calcium release from intracellular organelle stores involving calcium pumps or channels and calcium entry and activity of calcium-permeant ionotropic receptors or calcium-coupled GPCR, or plasma membrane calcium channels.

> Experience with different cellular modelscell linesprimary cultures from specific brain regions

> Kinetic monitoring of intracellular calcium using either FLIPR Tetra® or automated high-content imaging

> Deep knowledge of pharmacology principles and manipulations to target calcium signaling through a specific calcium channel or receptor, particularly in neuronal cells

FLIPR TETRA® CALCIUM ASSAYS

FLIPR Tetra® is particularly adapted to study calcium signaling in whole cell populations or cells predominantly present in the cell culture.

> High-throughput: simultaneous acquisition of Ca2+ signal in all wells of microplates

> Whole cell population analysis: when mean response reflects single-cell response

HIGH-CONTENT IMAGING FOR SINGLE-CELL CALCIUM ASSAYS

High-content imaging enables to monitor intracellular calcium at single-cell level, allowing accurate measurements:

> in heterogeneous cell populations, based on disctinctive Ca2+ response

> Segmentation-based single-cell analysis

 

 Learn about additionnal assays in the field of CNS disorders