ANGIOGENESIS

Fluofarma offers to identify and characterize compounds modulating angiogenesis through a quantitative in vitro endothelial tube formation assay. In addition, a range of molecular assays is available to explore the mechanism of action of compounds either activating or inhibiting angiogenesis.

IN VITRO ANGIOGENESIS ASSAY

Our assay is based on the ability of endothelial cells to form capillary-like tubes in a co-culture model of fibroblasts and GFP-expressing HUVECs:

> Cells cultured in a specifically designed medium
> Angiogenesis is monitored by live content time-lapse imaging for the identification and visualization of HUVEC tube formation
> Tube formation is analyzed using an appropriate angiogenesis module, for robust determination of the total tube length
> Our angiogenesis assay enables to characterize compounds' effects in both dose- and time-dependent manner.

SIGNALING PATHWAY ASSAYS RELATED TO ANGIOGENESIS

To better understand the mechanim(s) of action of angiogenic modulators, we offer to monitor the status of specific signaling pathways related to angiogenesis, such as H1F1α, Smad, P38, PI3K...

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