LIVE-CELLS ANALYSIS

LIVE-CELLS ANALYSIS

Besides endpoint measurements by high-content imaging, Fluofarma offers to monitor live cells over several days by time-lapse imaging. In fact, kinetic measurements allow to accurately determine the toxicity and efficacy profiles of lead compounds. In addition to facilitating dose selection and adjusting treatment conditions, live content imaging is implemented internally at Fluofarma to optimize our cell differentiation protocols.

KEY ADVANTAGES OF LIVE-CONTENT IMAGING

 > No more hidden drug effects: kinetic pharmacological profiling in live cells over several days

> Accurate dose-responses: determination of time-dependent EC50 values

> Less wells, more data: a single well provides the whole kinetic data of a given condition

Kinetic analysis of colistin-incuded cytolysis in HK-2 cell line.

Kinetic analysis of colistin-incuded cytolysis in HK-2 cell line.

Primary hippocampal neurons plated and treated with BDNF. Neuritic growth was then followed kinetically by label-free time-lapse imaging over 72h. Phase contrast images were segmented and treatment effect was evaluated on quantitative neurite parameters. BDNF stimulates neuritic growth in hippocampal neurons only after 48h.

Primary hippocampal neurons plated and treated with BDNF. Neuritic growth was then followed kinetically by label-free time-lapse imaging over 72h. Phase contrast images were segmented and treatment effect was evaluated on quantitative neurite parameters. BDNF stimulates neuritic growth in hippocampal neurons only after 48h.

Dose-dependent cytotoxic effects of arsenic trioxide on primary rat hepatocytes over 24hours.

Dose-dependent cytotoxic effects of arsenic trioxide on primary rat hepatocytes over 24hours.

FLUOFARMA'S ADDED VALUE

> Combination of live content & high-content imaging to investigate the molecular underpinnings of cellular response (signaling pathway analysis in the context of drug mechanism of action studies)

> Kinetic toxicity profiling in primary cell cultures : primary rat or human hepatocytes, primary human renal proximal tubule epithelial cells, primary rodent neuronal cultures, ...

> Kinetic monitoring of cell differentiation (cell lines and stem cells)

> In-house capacities for image segmentation & data analysis: custom algorithms for image analysis and feature extraction, and proprietary data analysis and visualization tool:Cytosurfer®

TECHNICAL FEATURES

INSTRUMENT INCUCYTE™ Kinetic Imaging System
FORMAT 96- / 384-well plates
MULTIPLEXING phenotypic features + fluorescent signals
BIOLOGICAL SAMPLES live cell cultures