PRIMARY CULTURES & STEM CELLS

Our high-content analysis services are supported by more than 10-year experience in the development of primary cell cultures. Directly isolated from fresh organs and grown in vitro, primary cell cultures retain most in vivo features, such as endogenous protein expression levels. Those highly predictive in vitro models are therefore used at Fluofarma to accurately determine the pharmacological profile of lead compounds: efficacy, toxicity and mechanism(s) of action.

PROFICIENCY IN PRIMARY CELL CULTURE

Neurons (MAP2, green) and astrocytes (GFAP, red) identified in mature mouse striatal culture.

Neurons (MAP2, green) and astrocytes (GFAP, red) identified in mature mouse striatal culture.

> High-reproducibility: isolations are extensively characterized to ensure uniform experimental conditions

> Flexible cell culture configuration: monolayer, sandwich or 3D spheroid cultures

> High-throughput format

> Generation of in vitro disease models based on pharmacological treatment or genetic modification

IN-HOUSE RODENT PRIMARY CELL CULTURES

Starting from our client's project, we develop fit for purpose organ-specific primary cell cultures from embryonic and newborn rodents. Here is an indicative list of the primary cultures already performed in microplate format:

PRIMARY NERVE CELL CULTURES OTHER PRIMARY CELL CULTURES
Primary cortical cell cultures
Primary hippocampal cell cultures
Primary hypothalamic cell cultures
Primary mesencephalic cell cultures
Primary striatal cell cultures 
Primary vomeronasal organ culture
Primary spinalneuron cultures		 Primary hepatocytes

Primary cardiomyocytes

Anterior pituitary cell cultures

STEM CELLS & CRYOPRESERVED HUMAN PRIMARY CELLS

Human umbilical vein endothelial (HUVEC) cells forming blood vessel-like tubes (in green). A segmentation mask (in red) enables the quantification of neo-vascular trees.

Human umbilical vein endothelial (HUVEC) cells forming blood vessel-like tubes (in green). A segmentation mask (in red) enables the quantification of neo-vascular trees.

Fluofarma offers to source cryopreserved human primary cultures, obtained from certified sources, following strict ethical standards.

Different isolations are first characterized to determine the best donors and reduce variability between cultures.

Below is an indicative list of the human primay cell cultures already performed in microplate format:

> Blood cells
> Endothelial cells
> Dermal and epidermal cells
> Hepatocytes
> Renal proximal tubule cells