3D SPHEROID CULTURE

Many cells cultured in scaffold-free systems spontaneously form spheroidal aggregates. The resulting in vitro 3D models recapitulate in vivo features such as cell-cell interactions, cell polarity, anchorage independent growth, as well as hypoxia and metabolic gradients in the case of tumor spheroids. Fluofarma has developed dedicated high-content analysis methodologies to fully investigate the effects of drug candidates at the single-cell level in 3D microtissues.

> Development and generation of mono- and multi-cell type spheroids using cell lines and primary cells

> Reproducible in 96-well screening format

> 1 spheroid = one well = one experimental condition

> High-throughput single-cell analysis of individual spheroids

> Cell proliferation, cell cycle and apoptosis functional assays

> Automated quantitative imaging of spheroids microarrays

> Cytometric image-based analysis at single-cell resolution

> Multiplex analysis of intracellular biomarkers: expression level, subcellular distribution, and subpopulation analysis