Neuritic growth is a key readout to monitor neuronal function and health. Fluofarma has developed a range of in vitro neurite outgrowth assays, to be performed in a variety of cell lines or primary cell cultures, in order to identify and characterize compounds inducing neuroprotection, neuroregeneration, neurotoxicity, or modulating neuritogenesis.
> Kinetic & label-free neurite outgrowth monitoring performed by live-content imaging
> Segmentation of phase-contrast images & quantification of total neuritic network
> Neuroprotective or neurotoxic effects characterized over several days
Primary cortical neurons are plated and treated with Taxol for 10 days. Neuritic growth is then followed kinetically by phase contrast time-lapse imaging over several days. Phase contrast images are segmented and treatment effect is evaluated on representative neurite parameters such as branch points. |
> Automated high-content imaging, using confocal microscopy
> Single-cell image analysis: neuritic growth can be ascribed to single cells using segmentation mask parameters
> Multiplexed detection with neuronal markers enables to measure neurite outgrowth in specific subpopulations
In addition to direct measurements of neuritogenesis, protein markers such as actin, tubulin, GSK3B, ERK, Jun, c-Fos, etc..., can be quantified at the single-cell level by high-content imaging to study specific signaling pathways/mechanisms of action.
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