γ secretase
γ secretase are proteases that cleave substrates such as the amyloid precursor protein (APP) and the Notch proteins. Abnormal proteolysis of APP is involved in the pathogenesis of Alzheimer's Disease (AD) and overactive Notch signaling plays an oncogenic role in a variety of cancers. γ Secretase have emerged as promising targets for drug development in the treatment of AD and cancer.
Gamma secretase biosensor
Fluofarma’s proprietary γ secretase biosensor is a chimeric construct based on a GFP-like reporter linked to C99 protein (β amyloid precursor) specifically cleaved by γ secretase. Our specific biosensor allows the assessment of γ secretase activity as it induces relocation of the fluorescence signal from the inner plasma membrane into the cytoplasm.
Biosensor in action
When γ secretase is not active, the fluorescent biosensor is located to the inner plasma membranes and its fluorescence remains in the cell after plasma membrane permeabilization. In contrast, when γ secretase is active, the biosensor is cleaved; the GFP-like protein relocates to the cytoplasm and the fluorescence is lost after the permeabilization of the plasma membrane. The number of fluorescent cells in a given condition provides a quantitative measure of γ secretase activity.
Breaking news
High Content Analysis 2012
Fluofarma will be attending the High Content Analysis 2012 in San Francisco, from January 9th to 12th. We look forward to seeing you at our booth (n° 34) and your are welcome the 11th at the « Luncheon Technology Showcase: High-Content Data Analysis » where Fluofarma will present CytoSurfe®r. An innovative and intuitive software application for High content image analysis.Read more [+]
Scientific Publication
Fluofarma is glad to announce a new scientific publication in the journal "The American Journal of Pathology".Read more [+]