Biosensors DAPs

A unique combination: DAPs & High-throughput Flow Cytometry

Combined with high-throughput flow cytometry Fluofarma DAPs technology captures molecular events at single-cell level on a large cellular population, and constitutes a robust alternative tool to quantitative and high-content imaging.

Added values

This unique technology provides the highest precision for quantifying the effects of compounds on molecular events such as enzyme activities and accessibility and protein intracellular compartmentalization.
Fluofarma DAPs technology offers a unique opportunity to monitor specific molecular events involved in a range of pathophysiological processes such as cancer, and neurodegenerative diseases.

Principles

Fluofarma's probes, based on fluorescent fusion proteins, are reporter substrates for specific cellular events. The biosensing proteins are stably expressed in human cell lines and convert a biological response into a fluorescent signal which can be measured by flow cytometry or cellular imaging. Hence, fluorescent changes in living cells expressing a specific biosensor are linked to the corresponding, specific biological event.

Applications

The versatility of DAPs provides Fluofarma Bioengineering division with a unique tool to develop new assays and/or uncover the action mechanisms of compounds.
Fluofarma Screening division offers several off-the-shelf assays based on DAPs allowing to monitor the activity of Caspase 3, Calpain and γ secretase specific activities.
Other biosensors are currently under development.