Assays are listed alphabetically by cellular functions and are cross-listed in all relevant general categories
| Cell Death (Cytotoxicity, Apoptosis, Autophagy…) | Inflammation & Cell Stress |
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Activated NF KappaB/p65 (phosphorylation on Ser529 ) Autophagy Autophagy and Poly-Ubiquitin Detection Calpain activity (Fluofarma biosensor) Caspase 3/7 activation (FAM-DEVD-FMK peptide) Caspase 3/7 activity (Fluofarma biosensor) Caspase 9 Activation Cell cycle Cell cycle on synchronized cells Cell proliferation (EdU) Cell proliferation index Cell senescence CHOP/GADD153 Detection c-Jun Activation Cleaved PARP Detection Clonogenicity (Flow cytometry) Cytochrome c release Cytolysis Cytotoxicity ERK activation (pERK1/2) Heme Oxygenase 1 and Phospho-p38 Activation Histone H3 acetylation (Lys9) Hsp27 and Phospho-Hsp27 Detection Hsp27 Detection Inositol trisphosphate receptor channel Intracellular calcium homeostasis Intracellular Glutathione (GSH) content Intracellular neutral lipid accumulation Intracellular neutral lipid and phospho-lipid accumulation Intracellular phospho-lipid accumulation Intracellular reactive oxigen species Ku70/80 Activation MDM2 and p53 Detection MDM2 Detection Micronuclei Mitochondrial membrane potential Neurite outgrowth NFkB Activation NFkB and c-Jun Activation p21 p38 activation (phospho-p38) p53 activation p53 and p21 Detection Assay P-glycoprotein (P-gp) activity Phospho-4E-BP1 Detection Phospho-ATM Activation Phospho-ATM and p53 Activation Phospho-Chk2 Activation Phospho-c-Jun and Phospho-JNK Detection Phospho-GSK-3 Detection Phospho-Hsp27 Detection Phospho-JNK Detection Phospho-mTOR Phospho-p53 and p53 Activation Phosphophorylated Akt substrates (motif R-X-R-X-X-S*/T* ) Phosphophorylated PKA substrates (motif R-R-X-S*/T*) Phosphorylated ATM/ATR substrates (motif Hyd-S*/T*-Q) Phosphorylated CDK substrates (motif K/R-S*-P-X-K/R) Phosphorylated MAPK/CDK substrates (motif T*-P) Phosphorylated PKC substrates (motif R/K-X-S*-Hyd-R/K ) Plant cell health status (ecotoxicology) Poly-Ubiquitin Detection |
Activated NF KappaB/p65 (phosphorylation on Ser529 ) ATF-2 Activation Autophagy Autophagy and Poly-Ubiquitin Detection Beta-Catenin Activation Calpain activity (Fluofarma biosensor) Caspase 3/7 activation (FAM-DEVD-FMK peptide) Caspase 3/7 activity (Fluofarma biosensor) Caspase 9 Activation Cell cycle Cell cycle: BrdU - Histone H3 Cell cycle on synchronized cells Cell migration Cell proliferation (EdU) Cell proliferation index Cell senescence CHOP/GADD153 Detection c-Jun Activation Cleaved PARP Detection Clonogenicity (Flow cytometry) COX-2 Activation Cyclin B1 Activation Cytochrome c release Cytolysis Cytoskeletal Rearrangement: F-actin Cytoskeletal Rearrangement: F-actin and Tubulin Cytoskeletal Rearrangement: Tubulin Cytotoxicity DNA double strand breaks Dopamine metabolism (DOPAC/DA ratio) Endothelial tube formation (in vitro angiogenesis) ERK activation (pERK1/2) FADH2 FOXO1A Activation FOXO3a gamma secretase Glucocorticoid Receptor translocation Heme Oxygenase 1 Activation Heme Oxygenase 1 and Phospho-p38 Activation HIF-1 alpha HIF-1 alpha, Phospho-CREB, FOXO3a Histone H3 acetylation (Lys9) Hsp27 and Phospho-Hsp27 Detection Hsp27 Detection Hsp60 and Hsp90-beta Detection Hsp60 Detection Hsp70 Detection Hsp70 and Hsp90-alpha Detection Hsp90Detection Immunophilin FKBP52 Detection iNOS Activation Inositol trisphosphate receptor channel Intracellular calcium homeostasis Intracellular Glutathione (GSH) content Intracellular neutral lipid accumulation Intracellular neutral lipid and phospho-lipid accumulation Intracellular phospho-lipid accumulation Intracellular reactive oxigen species Ku70/80 Activation MDM2 and p53 Detection MDM2 Detection Micronuclei Mitochondrial membrane potential Mitotic index: Phospho-histone H3 MnSOD and Phospho-H2AX Induction MnSOD Induction NADH,H+ and FADH2 NADH,H+ Neurite outgrowth NFAT Activation NFkB Activation NFkB and c-Jun Activation Quantification of dopaminergic neurons (TH) p21 p38 activation (phospho-p38) p53 activation p53 and p21 Detection Assay P-glycoprotein (P-gp) activity Phospho-4E-BP1 Detection Phospho-ATM Activation Phospho-ATM and p53 Activation Phospho-Chk2 Activation Phospho-c-Jun and Phospho-JNK Detection Phospho-CREB Phospho-GSK-3 Detection Phospho-Hsp27 Detection Phospho-JNK Detection Phospho-mTOR Phospho-p53 and p53 Activation Phosphophorylated Akt substrates (motif R-X-R-X-X-S*/T* ) Phosphophorylated PKA substrates (motif R-R-X-S*/T*) Phospho-PLK1 Activation Phospho-Rb Activation Phosphorylated ATM/ATR substrates (motif Hyd-S*/T*-Q) Phosphorylated CDK substrates (motif K/R-S*-P-X-K/R) Phosphorylated MAPK/CDK substrates (motif T*-P) Phosphorylated PKC substrates (motif R/K-X-S*-Hyd-R/K ) Phospho-S6 Detection PKA Activation PKA and Phospho-CREB Activation PKC-alpha Activation Plant cell health status (ecotoxicology) Poly-Ubiquitin Detection Smad3 Smad3 and Phospho-CREB STAT1 STAT2 STAT3 Synaptogenesis: MAP2, PSD95, Synaptophysin Tau phosphorylation (Tau-P Ser199 rate) Topoisomerase II activity |
| Genotoxicity, DNA Damage & Repair | Cell Signaling & Transcription Factors |
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Cell cycle Cell cycle on synchronized cells Cell proliferation (EdU) Cell proliferation index Cell senescence Cleaved PARP Detection Clonogenicity (Flow cytometry) DNA double strand breaks Intracellular reactive oxigen species Ku70/80 Activation MDM2 and p53 Detection MDM2 Detection Micronuclei MnSOD and Phospho-H2AX Induction MnSOD Induction p21 p53 activation p53 and p21 Detection Assay Phospho-ATM Activation Phospho-ATM and p53 Activation Phospho-Chk2 Activation Phospho-p53 and p53 Activation Topoisomerase II activity |
Activated NF KappaB/p65 (phosphorylation on Ser529 ) ATF-2 Activation Autophagy Autophagy and Poly-Ubiquitin Detection Beta-Catenin Activation Calpain activity (Fluofarma biosensor) Caspase 9 Activation Cell migration Cell senescence CHOP/GADD153 Detection c-Jun Activation Clonogenicity (Flow cytometry) Dopamine metabolism (DOPAC/DA ratio) ERK activation (pERK1/2) FADH2 FOXO1A Activation FOXO3a gamma secretase Glucocorticoid Receptor translocation HIF-1 alpha HIF-1 alpha, Phospho-CREB, FOXO3a Histone H3 acetylation (Lys9) Inositol trisphosphate receptor channel Intracellular calcium homeostasis Intracellular Glutathione (GSH) content Intracellular neutral lipid accumulation Intracellular neutral lipid and phospho-lipid accumulation Intracellular phospho-lipid accumulation Intracellular reactive oxigen species MDM2 and p53 Detection Mitochondrial membrane potential NADH,H+ and FADH2 NADH,H+ Neurite outgrowth NFAT Activation NFkB Activation NFkB and c-Jun Activation p21 p38 activation (phospho-p38) p53 activation p53 and p21 Detection Assay Phospho-ATM Activation Phospho-ATM and p53 Activation Phospho-Chk2 Activation Phospho-c-Jun and Phospho-JNK Detection Phospho-CREB Phospho-GSK-3 Detection Phospho-JNK Detection Phospho-mTOR Phospho-p53 and p53 Activation Phosphophorylated Akt substrates (motif R-X-R-X-X-S*/T* ) Phosphophorylated PKA substrates (motif R-R-X-S*/T*) Phosphorylated ATM/ATR substrates (motif Hyd-S*/T*-Q) Phosphorylated CDK substrates (motif K/R-S*-P-X-K/R) Phosphorylated MAPK/CDK substrates (motif T*-P) Phosphorylated PKC substrates (motif R/K-X-S*-Hyd-R/K ) PKA Activation PKA and Phospho-CREB Activation PKC-alpha Activation Poly-Ubiquitin Detection Smad3 Smad3 and Phospho-CREB STAT1 STAT2 STAT3 Tau phosphorylation (Tau-P Ser199 rate) |
| Cell Cycle & Proliferation | Morphology & Phenotypic Changes |
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Cell cycle Cell cycle: BrdU - Histone H3 Cell cycle on synchronized cells Cell proliferation (EdU) Cell proliferation index Cell senescence Clonogenicity (Flow cytometry) Cyclin B1 Activation ERK activation (pERK1/2) Histone H3 acetylation (Lys9) Intracellular reactive oxigen species Ku70/80 Activation Mitotic index: Phospho-histone H3 p21 p38 activation (phospho-p38) p53 activation Phospho-Chk2 Activation Phospho-p53 and p53 Activation Phosphophorylated Akt substrates (motif R-X-R-X-X-S*/T* ) Phosphophorylated PKA substrates (motif R-R-X-S*/T*) Phospho-PLK1 Activation Phospho-Rb Activation Phosphorylated ATM/ATR substrates (motif Hyd-S*/T*-Q) Phosphorylated CDK substrates (motif K/R-S*-P-X-K/R) Phosphorylated MAPK/CDK substrates (motif T*-P) Phosphorylated PKC substrates (motif R/K-X-S*-Hyd-R/K ) Plant cell health status (ecotoxicology) |
Cell migration Cell senescence Clonogenicity (Flow cytometry) Cytoskeletal Rearrangement: F-actin Cytoskeletal Rearrangement: F-actin and Tubulin Cytoskeletal Rearrangement: Tubulin Endothelial tube formation (in vitro angiogenesis) Micronuclei Neurite outgrowth NeuroToxicity: Gliosis Quantification of dopaminergic neurons (TH) Synaptogenesis: MAP2, PSD95, Synaptophysin |
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