Fluofarma is glad to announce a new scientific publication in the journal
"Analytical Chemistry":
Recombinant Differential Anchorage Probes that
Tower over the Spatial Dimension of Intracellular Signals for High Content
Screening and Analysis
Laura Schembria, Marion Zaneseb,
Gaelle Depierre-Plinetb, Muriel Petitb,
Assia Elkaoukabi-Chaibib, Loic Tauzinb,
Cristina Floreana,c, Lydia Lartiguea,
Chantal Medinaa, Christophe Reya,
Francis Bellocd, Josy Reiffersa,
François Ichasa,b and Francesca
De Giorgi*a,b
a INSERM U916, Institut Bergonié, 229 cours
de l'Argonne, 33076 Bordeaux, France
b Fluofarma, 2 rue Robert Escarpit, 33600
Pessac, France
c Università di Padova, 35121 Padova, Italia
d CHU de Bordeaux, Hôpital du Haut Lévèque,
33600 Pessac, France
* Corresponding author : francesca.degiorgi-ichas@inserm.fr
Abstract
Recombinant fluorescent probes allow the detection of molecular
events inside living cells. Many of them exploit the intracellular space to
provide positional signals and, thus, require detection by single cell
imaging. We describe here a novel strategy based on probes capable of encoding
the spatial dimension of intracellular signals into “all-or-none” fluorescence
intensity changes (differential anchorage probes, DAPs). The resulting signals
can be acquired in single cells at high throughput by automated flow
cytometry, (i) bypassing image acquisition and analysis, (ii) providing a
direct quantitative readout, and (iii) allowing the exploration of large
experimental series. We illustrate our purpose with DAPs for Bax and the
effector caspases 3 and 7, which are keys players in apoptotic cell death, and
show applications in basic research, high content multiplexed library
screening, compound characterization, and drug profiling.
Analytical Chemistry advance online publication October 29, 2009; doi: 10.1021/ac9015227
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