Fluofarma _{understanding drugs}

Fluofarma is a preclinical Contract Research Organization (CRO) which delivers
High Content Analysis services.

Specialized in assay development & high-content screening, Fluofarma designs
and performs cell-based assays, and provides biomarker analysis services in
tissue & whole blood samples, using automated high-content imaging & flow
cytometry technologies.

► Cell-based assays & Screening

■ HCA, HCS, HTS for:

o Target validation
o Primary & secondary screenings
o Lead optimization
o Mechanism of action studies / Drug profiling
o Predictive Toxicity

■ Custom design & development of cell-based assays
■ “Off-the-shelf” : ≈ 150 validated in vitro cellular assays

► Quantitative tissue imaging & biomarkers

■ Transfer of HCA methodologies to tissue sections,
tissue microarrays (TMA) and whole blood for:

o Preclinical Target hitting/ Mechanism of action in vivo
o Preclinical Toxicology in vivo
o Patient stratification in clinical trials
o Biomarkers & Theragnostics

► Image & data analysis

■ Custom development of software for:

o Image acquisition /segmentation / analysis
o Analysis & management of large data loads (screening, population studies)
o Kinetic data quantification
o Bioinformatics, predictive models

■ “Off-the-shelf” software: Cytosurfer

CytoSurfer® is a ready to use image data analysis software for High Content Screening
(HCS).

CytoSurfer® efficiently bridges flow cytometry and image analysis visualizing data through
interactive multidimensional scatter plots which allow to identify and visualize subpopulations
of cells.

► In one click with Cytosurfer® you can:

■ Select and independently analyze different cell populations
■ Go back and forth between graphs and original cell images
■ Load plate layouts (96 or 384 wells) to regroup and analyze well replicates
■ Analyze data statistically and generate comprehensive reports

Please visit the CytoSurfer® website for more information and obtain a demo version.